View Of A Streamlined Crispr Cas9 Approach For Fast Genome Editing In Toxoplasma Gondii And Besnoitia Besnoiti Journal Of Biological Methods
PAM (seed region) are especially important for target site recognition In many cases, offtarget mutations happen at sites where the gRNA seed region has no mismatches but the nonseed region does 3, 15 To knock out a specific region in the genome, such offtarget effects should be avoided Reducing such risk is espe cially important when we consider further application ofGRNA targeting region of interest gRNA targeting gene promoter elements gRNA targeting gene promoter elements Table 2 Plasmid Vector Expression System Components Applica on Constitutive or inducible Cas9 Constitutive or inducible gRNA Reporter/selection marker Expression of Cas9 and gRNA 3 wwwgenscriptcom Modified recombinant AAV particles are attractive for transduction
